Generally, once the collection of the semen is done, it is analyzed at the laboratory and diluted with the extender at a temperature similar to that of the ejaculate (between 33-37ºC) . The difference of temperature should be between ± 1 or 2 ºC , because of the reasons mentioned in the previous entry. It is 1-STEP ISOTHERMAL DILUTION.
With the evolution of the swine sector, new working methods and needs have arisen in boar studs, such as satellite laboratories where ejaculates from different locations are processed. Then, they must be transported with a previous dilution (semen ratio 1: 1 or 1: 2) for its preservation and protection during the journey to the laboratory and final processing.
Another change has been the increase in the size of boar studs and the rise in the number of doses to be produced on certain days, (usually on Sundays or Mondays and Thursdays). It causes that the doses produced from the last collected ejaculates do not have enough time to reach the conservation temperature, prior to transport to the destination farms. As we commented in the previous entry, we must make the sperm reach the temperature of 15-17ºC, since it is in this range that the metabolic activity of the sperm is minimized. So it is essential.
Although the transportation may be cool and/or with controlled temperature, it is very complicated that if the doses have not previously reached the target temperature, they do so during the journey. These chambers maintain the temperature, but they do not make it decrease, since that its capacity is much smaller than the preserving chambers that exist in the boar studs. If the delivery was made using parcel service, the scenario would be even worse.
As a result, new forms of work have emerged. They allow us to face the challenges that appear in insemination centers:
- transportation of ejaculates to the processing centers
- fast reduction of temperature prior to transport